解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 274-278.doi: 10.3969/j.issn.0529-1356.2013.02.025

• 组织学胚胎学发育生物学 • 上一篇    下一篇

葛瑞林对心肌梗死致心力衰竭大鼠保护作用及生长分化因子-15的影响

田国忠*  魏罡  李梅秀  钟震亚  朱金玲  李艳君  杨宇  魏伟.   

  1. 佳木斯大学基础医学院解剖学教研室，黑龙江 佳木斯 154007
  • 收稿日期:2012-05-15 修回日期:2012-06-12 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 魏罡 E-mail:wg0912@163.com
  • 基金资助:

    黑龙江省教育厅研究生创新科研项目（YJSCX2011-373HLJ）；黑龙江省自然科学基金（D2006-24）。;省自然科学基金资助项目;自然科学基金资助项目

Effects of Ghrelin on the protective effect and influence of growth differentiation factor-15 in the rat with the heart failure induced by myocardial infarction

TIAN Guo-zhong* WEI Gang LI Mei-xiu ZHONG Zhen-ya ZHU Jin-ling LI Yan-jun YANG Yu WEI Wei    

  1. Department of Anatomy, Basic Medical College, Jiamusi University, Helongjiang Jiamusi 154007, China
  • Received:2012-05-15 Revised:2012-06-12 Online:2013-04-06 Published:2013-04-06

摘要：

目的 制作葛瑞林（Ghrelin）早期干预心肌梗死致心力衰竭(HF)大鼠模型，探讨生长分化因子-15(GDF-15)表达水平与HF程度相关性，为HF临床治疗奠定理论学基础。方法 80只Wistar大鼠于造模成活6h后被随机分为假手术（A）组（n =18)、HF模型(B)组（n =27)和HF模型干预(C)组（n =27)，通过酶联免疫吸附（ELISA）及实时定量 PCR，于造模后3d、1周、2周、4周、8周分别检测各组血中GDF-15表达变化及2周、4周、8周末3组HF大鼠心肌组织中GDF-15 mRNA表达水平的改变；电镜下观察各组大鼠心肌组织超微结构，并均于处死前行B型超声检测心功能。结果 B组大鼠血GDF-15及心肌组织GDF-15 mRNA表达水平明显高于C组，低于A组，差异具有统计学意义（P ＜0.001）；8周末超声检测结果显示，B组大鼠较A、C两组大鼠心脏增大明显，左室舒张及收缩末期内径均明显增加，差异具有统计学意义（P ＜0.001）；B组大鼠左室舒张末期室间隔厚度、左室后壁厚度及射血分数值均较A、C两组大鼠显著降低，差异具有统计学意义（P ＜0.001）；此外，心肌超微结构显示C组较B组改变明显。结论 Ghrelin早期干预HF大鼠能改善心功能，降低死亡率；GDF-15表达水平能反映HF病变程度，并可作为辅助检测HF新的标记物。

关键词: 葛瑞林 ,  心力衰竭 ,  心肌梗死 ,  生长分化因子-15 , 酶联免疫吸附法 ,  B型超声 , 电镜 , 大鼠

Abstract:

Objective The previous experiment showed that Ghrelin intervened in myocardial infarction leading to heart failure(HF) in rats at the early stage. The aim of this paper is to further investigate the correlation between growth differentiation facto-15 (GDF-15) and HF degree, which might lay a theoretical foundation of HF clinical treatments. Methods Eighty Wistar rats survived 6 hours in the modeling were randomly divided into sham operation(group A)( n =18), HF model (group B)( n =27), HF model of intervention(group C)( n =27). The blood GDF-15 expression level in 3 days，1 week，2 weeks，4 weeks and 8 weeks after surgery and the GDF-15 mRNA expression level in HF myocardial tissue in 2 weeks, 4 weeks and 8 weeks were tested by ELISA and real-time PCR. The rat myocardial ultrastructure was observed by electron microscope. Cardiac function was checked by B ultrasonic before executing the rats. Results Group B blood GDF-15 and myocardial tissue GDF-15 mRNA expression level were significantly higher than group C, but lower than group A. The difference was statistically significant( P <0.001).Ultrasonic testing results showed that group B heart increased significantly,left ventricular systolic and diastolic inner diameter increased obviously than group A and C in the 8 weeks. The difference was statistically significant ( P <0.001). Group B end-diastolic ventricular septal thickness, left ventricular thickness and left ventricular ejection fraction were significantly reduced than group A and C; In addition, myocardial ultrastructure shows group C change significantly than group B. Conclusion Ghrelin early intervention in HF in rats can improve heart function and reduce mortality; GDF-15 expression level can reflect HF lesions degree, and can be a new marker as auxiliary detection of HF.

Key words:  Ghrelin , Heart failure , Myocardial infarction , Growth differentiation factor-15 , Enzyme-linked immunosorbent assay ,  B-mode ultrasound , Electron